What is the running buffer for Bis-Tris

In chemical and biological experiments, buffer solutions can maintain the pH stability of the solution and ensure experimental results. Bis Tris (bis (2-hydroxyethyl) amino (trihydroxymethyl) methane) is a commonly used buffer, with a buffering range typically between pH 5.8-7.2. This range makes it very useful in many biochemical and molecular biology experiments, especially in experiments that require neutral or slightly acidic conditions. At the same time, Bis Tris exhibits good stability over a wide temperature range. It can maintain the pH stability of the solution under different temperature conditions, which is very important for experiments that need to be conducted at different temperatures.

Bis Tris powder

Preparation method of Bis Tris running buffer

1. Determine the required buffer concentration and pH value: Before preparing the Bis Tris running buffer zone, it is necessary to determine the required buffer concentration and pH value. This can be determined based on experimental requirements and the characteristics of Bis Tris.

2. Calculate the required amount of Bis Tris and other reagents: Based on the required buffer concentration and pH value, the required amount of Bis Tris and other reagents can be calculated.

3. Dissolve Bis Tris and other reagents: Dissolve the calculated Bis Tris and other reagents in an appropriate amount of deionized water and stir evenly. During the dissolution process, attention should be paid to the order of adding reagents and stirring speed to ensure that the reagents can be fully dissolved.

4. Adjust pH value: Use a pH meter or other pH measuring instrument to measure the pH value of the solution. If the pH value is not within the required range, acid or alkali can be used to adjust the pH value. When adjusting the pH value, it is necessary to slowly add acid or base and continuously stir the solution to ensure the accuracy of the pH value.

5. Constant volume and filtration: Dilute the pH adjusted solution to the desired volume, and then filter the solution using a filter to remove impurities and insoluble particles. The filtered solution can be stored in a suitable container for experimental use.

Preparation method of Bis Tris running buffer

2. Calculate the required amount of Bis Tris and other reagents: Based on the required buffer concentration and pH value, the required amount of Bis Tris and other reagents can be calculated.

Precautions for using Bis Tris

1. Storage conditions: Bis Tris should be stored in a cool, dry place, away from direct sunlight and high temperatures. During storage, attention should be paid to sealing and preserving to prevent moisture evaporation and impurities from entering.

2. Validity period: Its validity period is usually around 1-2 years, depending on the composition and storage conditions of the buffer solution. Before using the buffer, its expiration date should be checked to ensure that its quality meets the experimental requirements.

3. Avoid contamination: When preparing and using Bis Tris, contamination should be avoided. Use clean containers and reagents to avoid cross contamination. During the experiment, attention should be paid to preventing the buffer from being contaminated by other substances such as proteins, nucleic acids, enzymes, and other biomolecules.

Product packaging

The selection of the running buffer for Bis Tris is crucial for the success of the experiment. When selecting the running buffer, factors such as experimental requirements, buffer range, ion strength, and buffer capacity need to be considered. Hubei Xindesheng Material Technology Co., Ltd., as a manufacturer of Bis Tris and other biological buffering agents, can not only supply analytical grade raw materials, but also provide customized services to meet customer customization needs. If you have purchasing intentions, please feel free to click on the website to inquire about details and purchase at any time!

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